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Sulforhodamine B

Red fluorescent dye

Sulforhodamine B or Kiton Red 620 (C27H30N2O7S2) is a fluorescent dye with uses spanning from laser-induced fluorescence (LIF) to the quantification of cellular proteins of cultured cells. This red solid dye is very water-soluble.

Spectroscopy

The dye has maximal absorbance at 565 nm light and maximal fluorescence emission at 586 nm light. It does not exhibit pH-dependent absorption or fluorescence over the range of 3 to 10.

Applications

Sulforhodamine B is often used as a membrane-impermeable polar tracer or used for cell density determination via determination of cellular proteins (cytotoxicity assay).

References

"Sulforhodamine B sodium salt (CAS 3520-42-1)". Santa Cruz Biotechnology.
(1998). "Dual Emission Laser Induced Fluorescence for Direct Planar Scalar Behavior Measurements". Experiments in Fluids.
(2015). "Switchable Lipids: Conformational Change for Fast pH-Triggered Cytoplasmic Delivery". Angewandte Chemie International Edition.
(2006). "Sulforhodamine B colorimetric assay for cytotoxicity screening". Nature Protocols.

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