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NBD-TMA

NBD-TMA (2-(4-nitro-2,1,3-benzoxadiazol-7-yl)aminoethyl]trimethylammonium) is a small, positively charged (+1) fluorescent dye. It was also known as EAM-1 (N,N,N,-trimethyl-2[(7-nitro-2,1,3-benzoxadiazol-4-yl)amino]ethanaminium iodide) when it was briefly supplied by Macrocyclics Company as an iodide complex.

NBD-TMA has an excitation maximum at 458 nm and an emission maximum at 530 nm. It also has a smaller local excitation maximum around 343 nm. The molar extinction coefficient is about 13,000 cm−1M−1 and its overall effective fluorescence is about 1% that of fluorescein. It is only mildly sensitive to halide ion collision quenching.

NBD-TMA was designed as a probe for monitoring renal transport of organic cations. As a small, positively charged fluorophore, it has also seen use as a tracer for measuring gap junction coupling in cases of cation selective connexin channels.

References

References

  1. (2000). "NBD-TMA: A novel fluorescent substrate of the peritubular organic cation transporter of renal proximal tubules". Pflügers Archiv: European Journal of Physiology.
  2. (2005). "Connexin26 is responsible for anionic molecule permeability in the cochlea for intercellular signalling and metabolic communications". The European Journal of Neuroscience.
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fluorescent-dyes benzoxadiazoles ethyleneamines nitroarenes secondary-amines quaternary-ammonium-compounds
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