MTSL

Labeling

MTSL is attached to proteins by reaction with thiol groups. The reaction exploits standard reactivity of thiosulfate esters. Methanesulfinate (CH3SO2−) is the leaving group: :RSO2S-nitroxide + protein-SH → protein-S-S-nitroxide + RSO2H The heterodisulfide bond to the cysteine residue is robust, enabling site-directed spin labelling. The MTSL moiety will add 184.3 daltons to the mass of the protein or peptide to which it is attached. The cysteine can be introduced using site-directed mutagenesis, and hence most positions in a protein can be labelled.

Spectroscopy

In Nuclear magnetic resonance the introduction of the paramagnetic group increases the relaxation rate of nearby nuclei. Its presence can be detected as peak broadening and loss of intensity in peaks corresponding to nearby nuclei. Hence proximity can be inferred for all nuclei, that are affected. A major advantage of this method over traditional methods for obtaining distance restraints in protein NMR is the increased length, as paramagnetic relaxation enhancement can detect distances up to 25 Å (2.5 nm) as opposed to about 6 Å (0.6 nm) using the nuclear Overhauser effect. Spin labelling with MTSL is frequently used in investigation of residual structure in intrinsically unstructured proteins.

References

References

1. Christian Altenbach, Kyoung-Joon Oh, René J. Trabanino, Kálmán Hideg, Wayne L. Hubbell "Estimation of Inter-Residue Distances in Spin Labeled Proteins at Physiological Temperatures:  Experimental Strategies and Practical Limitations" Biochemistry, 2001, volume 40, pp 15471–15482. {{doi. 10.1021/bi011544w
2. Kenyon, G.L. and Bruice, T.W. (1977). Novel sulfhydryl reagents. Methods In Enzymology 47, 407-430. {{doi. 10.1016/0076-6879(77)47042-3
3. Berliner, L.J., Grunwald, J., Hankovszky, H.O., Hideg, K. (1982). A novel reversible thiol-specific spin label: papain active site labeling and inhibition. Analytical Biochemistry 119, 450-455. {{doi. 10.1016/0003-2697(82)90612-1