GcvB RNA

Targets of GcvB

GcvB has been shown to regulate a large number of genes in E. coli and Salmonella species. GcvB was shown to bind to Oppa and DppA which transport oligopeptides and dipeptides respectively. It has been shown to also regulate gltL, argT, STM, livK, livJ, brnQ, sstT and cycA which are involved in uptake of a variety of amino acids. GcvB RNA also is involved in regulating a variety of genes involved in amino acid biosynthesis such as ilvC, gdhA, thrL and serA. GcvB RNA binds PhoPQ mRNA, which encodes a two-component system involved in magnesium homeostasis.

Polymerisation

There is evidence that E. coli GcvB can form polymers. Native polyacrylamide gel electrophoresis was used to show a higher molecular weight band corresponding to a potential polymer. Transmission electron microscopy was then used to identify a filamentous structure for the polymer. However, the authors suggest that these long filaments are unlikely to be physiologically relevant. It was shown that a construct containing only the first 61 nucleotides including the first stem-loop was sufficient for polymerisation. Similar results were recently shown for the DsrA RNA. The physiological relevance of polymerisation is not known.

Species distribution

The GcvB RNA is found in a range of bacteria including:

• Escherichia coli
• Yersinia pestis
• Haemophilus influenzae
• Vibrio cholerae
• Shigella dysenteriae
• Salmonella typhimurium
• Klebsiella pneumoniae
• Photorhabdus luminescens
• Pasteurella multocida

References

References

1. Urbanowski, ML. (2000). "The gcvB gene encodes a small untranslated RNA involved in expression of the dipeptide and oligopeptide transport systems in Escherichia coli". Mol Microbiol.
2. (2006). "The Yersinia pestis gcvB gene encodes two small regulatory RNA molecules". BMC Microbiol..
3. (2009). "Small noncoding RNA GcvB is a novel regulator of acid resistance in Escherichia coli". BMC Genomics.
4. (2015-06-03). "Cross talk between ABC transporter mRNAs via a target mRNA-derived sponge of the GcvB small RNA". The EMBO Journal.
5. (August 2000). "The gcvB gene encodes a small untranslated RNA involved in expression of the dipeptide and oligopeptide transport systems in Escherichia coli". Mol. Microbiol..
6. (January 2009). "Role of the Escherichia coli Hfq protein in GcvB regulation of oppA and dppA mRNAs". Microbiology.
7. (November 2007). "A small RNA regulates multiple ABC transporter mRNAs by targeting C/A-rich elements inside and upstream of ribosome-binding sites". Genes Dev..
8. (January 2009). "The small RNA GcvB regulates sstT mRNA expression in Escherichia coli". J. Bacteriol..
9. (January 2009). "Role of the sRNA GcvB in regulation of cycA in Escherichia coli". Microbiology.
10. Vogel J. (January 2009). "A rough guide to the non-coding RNA world of Salmonella". Mol. Microbiol..
11. Coornaert, A. (Jan 2013). "Post-Transcriptional Control of the Escherichia coli PhoQ-PhoP Two-Component System by Multiple sRNAs Involves a Novel Pairing Region of GcvB.". PLOS Genetics.
12. (2009). "Auto-assembly as a new regulatory mechanism of noncoding RNA.". Cell Cycle.
13. Cayrol B. (2009). "Auto-assembly of E. coli DsrA small noncoding RNA: Molecular characteristics and functional consequences". RNA Biol.
14. (April 2008). "The role of the small regulatory RNA GcvB in GcvB/mRNA posttranscriptional regulation of oppA and dppA in Escherichia coli". FEMS Microbiol. Lett..
15. (May 2018). "Determination of the small RNA GcvB regulon in the Gram-negative bacterial pathogen Pasteurella multocida and identification of the GcvB seed binding region". RNA.