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Downstream promoter element

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In molecular biology, a downstream promoter element (DPE) is a core promoter element. Like all core promoters, the DPE plays an important role in the initiation of gene transcription by RNA polymerase II. The DPE was first described by T. W. Burke and James T. Kadonaga in Drosophila melanogaster at the University of California, San Diego in 1996. It is also present in other species including humans, but not Saccharomyces cerevisiae.

Together with the initiator motif (Inr), another core promoter element, the DPE is recognized by the transcription factor II D (TFIID) subunits TAF6 and TAF9. It has been shown that DPE-dependent basal transcription depends highly on the Inr (and vice versa) and on correct spacing between the two elements.

The DPE consensus sequence was originally thought to be RGWCGTG, It is located about 28–33 nucleotides downstream of the transcription start site.

Occurrence

It has been shown that the DPE is about as widely used as the TATA box in D. melanogaster. While a DPE was found in many promoters that do not contain a TATA box, there are also promoters that contain both a TATA box and a DPE.

The promoters of nearly all Hox genes of D. melanogaster, with the exception of the evolutionarily most recent genes, Ubx and Abd-A, contain a DPE motif and lack a TATA box. Drosophila promoters containing the DPE sequence include Abd-B, Antp P2, bride of sevenless, brown, caudal, E74, E75, engrailed, Gsα, labial, nmMHC, ras2, singed, stellate, and white. In organisms other than D. melanogaster, the promoter of the human and mouse IRF1 gene has been found to contain a DPE consensus sequence at the appropriate distance from the transcription start site. This promoter, too, does not contain a TATA box.

DPE has also been reported to play role in primitive Eukaryote Entamoeba histolytica.

Notes

References

(15 October 2002). "The RNA polymerase II core promoter: a key component in the regulation of gene expression". Genes & Development.
(15 March 1996). "Drosophila TFIID binds to a conserved downstream basal promoter element that is present in many TATA-box-deficient promoters.". Genes & Development.
(March 2010). "Regulation of gene expression via the core promoter and the basal transcriptional machinery". Developmental Biology.
(15 November 1997). "The downstream core promoter element, DPE, is conserved from Drosophila to humans and is recognized by TAFII60 of Drosophila". Genes & Development.
(March 2007). "Prevalence of the initiator over the TATA box in human and yeast genes and identification of DNA motifs enriched in human TATA-less core promoters". Gene.
(1 July 2000). "The Downstream Promoter Element DPE Appears To Be as Widely Used as the TATA Box in Drosophila Core Promoters". Molecular and Cellular Biology.
(15 October 2008). "Caudal, a key developmental regulator, is a DPE-specific transcriptional factor". Genes & Development.
(2019-03-12). "Transcriptomic analysis reveals novel downstream regulatory motifs and highly transcribed virulence factor genes of Entamoeba histolytica". BMC Genomics.

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