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Double-disk diffusion test

Laboratory test in microbiology

Laboratory test in microbiology

A double-disk diffusion test is a kind of disk diffusion test (to test for the effectiveness of an antimicrobial agent a disk infused with it is placed on a cultivated agar dish of bacteria to see if the antimicrobial agent in the disk inhibits further growth of the bacteria.)

The double-disc synergy test (DDST) utilizes two of these disks on the cultivated agar solution, either infused with a different antimicrobial solution.

This test was recommended the standard by the Clinical and Laboratory Standards Institute in 2004 for its use against MRSA. Testing for inducible clindamycin resistance is typically performed in strains of Staphylococcus, β-hemolytic streptococci, and Streptococcus pneumoniae that demonstrate erythromycin resistance and clindamycin susceptibility.{{cite report

References

References

  1. (2007). "Antimicrobial Resistance and Implications for the 21st Century". Springer Science & Business Media.
  2. (2005). "Antibiotics in Laboratory Medicine". Lippincott Williams & Wilkins.
  3. (2007). "MRSA". CRC Press.
  4. (2013). "Infectious Disease, an Issue of Primary Care Clinics in Office Practice". Elsevier Health Sciences.
  5. "Double Disk Diffusion Test (D-Test)".
  6. (18 April 2018). "The D-test for Macrolide-Inducible Resistance to Clindamycin". Tulane University School of Medicine.
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bacteriology staphylococcus
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