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Bialaphos

Bialaphos is a natural herbicide produced by the bacteria Streptomyces hygroscopicus and Streptomyces viridochromogenes. It is also known by the ISO common name bilanafos. Bialaphos is a protoxin and nontoxic as is. When it is metabolized by a plant, the glutamic acid analog glufosinate is released which inhibits glutamine synthetase. This results in the accumulation of ammonium and disruption of primary metabolism.

Bialaphos is made up of two alanine residues and glufosinate, and is commonly used as a selection marker in plants. Resistance plasmids include pGreenII 0229 and pGreenII 0229 62-SK. pGreenII 0229 is derived from pGreenII 0000, a nos-bar cassette has been inserted into the HpaI site of the left border, providing resistance to bialaphos or phosphinothricin during plant transformation selection. pGreenII 0229 62-SK is derived from pGreenII 0229, the LacZ blue/white cloning selection has been replaced with a 35S-MCS-CaMV cassette that allows the insertion of a gene of interest into a 35S overexpression cassette.

References

References

  1. (1986). "The bialaphos biosynthetic genes of ''Streptomyces hygroscopicus'': Molecular cloning and characterization of the gene cluster". MGG Molecular & General Genetics.
  2. "Compendium of Pesticide Common Names: Bilanafos". BCPC.
  3. (2011). "Modes of Action of Microbially-Produced Phytotoxins". Toxins (Basel).
  4. "Bialaphos as plant gene selector".
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herbicides organophosphinic-acids eukaryotic-selection-compounds group-10-herbicides
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