Acrydite

History

The idea of acrylamide-modified DNA was developed by T. Christian Boles, while working at Mosaic Technologies, a now-defunct biotechnology company located in Waltham, MA. The IP was licensed, along with a microarray technology ("ez-rays") to Matrix Technologies, of Hudson, NH, which is now part of Thermo Scientific. Acrydite-modified oligonucleotides can be obtained from vendors such as Integrated DNA Technologies (IDT).

Hybrigel

The first use of Acrydite was in a technology called Hybrigel. In Hybrigel, Acrydite-modified oligos are incorporated into a standard polyacrylamide gel system; as complementary ss nucleic acid moves past the immobilized Acrydite oligos, the complementary DNA is captured. Hybrigel-like technology is widely used as a DNA purification system, as in a DNA sequencing technology developed at Berkeley. Acrydite technology can also be used to purify DNA, as in the Pre-gen colon cancer test developed by Exact Sciences.

Polonys

Mitra and Church at Harvard Medical School developed a novel DNA sequencing technology based on Acrydite-modified probes; this technology is used by Agencourt.

Acrydite and PCR

Surprisingly, the Acrydite group survives PCR, so Acrydite-modified PCR products can be prepared and used as oligos. However, as is common with polymers that are longer than the persistence length, the coupling of Acrydite-modified PCR products is inefficient, as the ends of the molecules are not accessible.

Acrydite and Protein

Acrydite-modified oligonucleotides have been used to study protein DNA interactions

Acrydite and aptamer-functionalized molecularly imprinted polymers

Acrydite-modified aptamers (and also aptamers containing internal acrylamide modifications) have been used to make AptaMIPs, molecularly imprinted polymers that use polymerizable aptamers as monomers for the imprinting. AptaMIPs show enhanced target-binding properties relative to traditional MIPs.

Acrydite and DNA Computing

Experiments by Braich et al. solving the Satisfiability Problem have demonstrated that Acrydite can be used in DNA computing to implement the extraction operation. |chapter-url = http://www.usc.edu/dept/molecular-science/papers/fp-gel-baseddna600.pdf |display-authors = etal |access-date = 2006-03-28 |archive-url = https://web.archive.org/web/20061205034454/http://www.usc.edu/dept/molecular-science/papers/fp-gel-baseddna600.pdf |archive-date = 2006-12-05 |url-status = dead |display-authors=etal| citeseerx = 10.1.1.69.7051

References

References

1. BioTechniques. 1998 Sep;25(3):516-21. Mutation typing using electrophoresis and gel-immobilized Acrydite probes. Kenney M, Ray S, Boles TC.
2. Proc Natl Acad Sci U S A 2006 May 9; 103(19): 7240–7245. Microfabricated bioprocessor for integrated nanoliter-scale Sanger DNA sequencing
3. [http://www.flagshipventures.com/news/12132002p.html Flagship Ventures] {{webarchive. link. (October 17, 2006)
4. Nucleic Acids Res. 1999 Dec 15;27(24):e34. ''In situ'' localized amplification and contact replication of many individual DNA molecules. Mitra RD, Church GM.
5. BioTechniques. 2002 Apr;32(4):808-10, 812, 814-5. Acrylamide capture of DNA-bound complexes: electrophoretic purification of transcription factors.
6. (2015). "Generation of Novel Hybrid Aptamer-Molecularly Imprinted Polymeric Nanoparticles". Advanced Materials.