Surf Wiki
Save to docs
general/9-phenylfluorone-dyes

From Surf Wiki (app.surf) — the open knowledge base

6-Carboxyfluorescein

6-Carboxyfluorescein (6-FAM) is a fluorescent dye with an absorption wavelength of 495 nm and an emission wavelength of 517 nm. A carboxyfluorescein molecule is a fluorescein molecule with a carboxyl group added. They are commonly used as a tracer agents. It is used in the sequencing of nucleic acids and in the labeling of nucleotides.

Commercially available FAM is a mixture of two isomers, 5-FAM and 6-FAM, and the correct name is 5(6)-carboxyfluorescein.

The dyes are membrane-impermeant and can be loaded into cells by microinjection or scrape loading.{{cite web | access-date = 2006-08-26 | access-date = 2006-08-26 | url-access = subscription

Popular derivatives for cell tracing purposes are carboxyfluorescein diacetate succinimidyl ester (CFDA-SE) and carboxyfluorescein succinimidyl ester (CFSE).

References

References

  1. (2013). "Modeling the parameters for plasmodesmal sugar filtering in active symplasmic phloem loaders". Frontiers in Plant Science.
  2. (2019-04-01). "Direct Comparison of Leaf Plasmodesma Structure and Function in Relation to Phloem-Loading Type". Plant Physiology.
  3. (1999-05-01). "Temporal and spatial regulation of symplastic trafficking during development in Arabidopsis thaliana apices". Development.
Info: Wikipedia Source

This article was imported from Wikipedia and is available under the Creative Commons Attribution-ShareAlike 4.0 License. Content has been adapted to SurfDoc format. Original contributors can be found on the article history page.

9-phenylfluorone-dyes benzoic-acids dicarboxylic-acids phenylogous-carboxylic-acids triarylmethane-dyes
Want to explore this topic further?

Ask Mako anything about 6-Carboxyfluorescein — get instant answers, deeper analysis, and related topics.

Research with Mako

Free with your Surf account

Content sourced from Wikipedia, available under CC BY-SA 4.0.

This content may have been generated or modified by AI. CloudSurf Software LLC is not responsible for the accuracy, completeness, or reliability of AI-generated content. Always verify important information from primary sources.

Report