28S ribosomal RNA

Use in phylogeny

The genes coding for 28S rRNA are referred to as 28S rDNA. The comparison of the sequences from these genes are sometimes used in molecular analysis to construct phylogenetic trees, for example in protists, fungi, insects, arachnids, tardigrades, and vertebrates.

Structure

The 28S rRNA is typically 4000–5000 nt long.

Some eukaryotes cleave 28S rRNA into two parts before assembling both into the ribosome, a phenomenon termed the "hidden break".

Databases

Several databases provide alignments and annotations of LSU rRNA sequences for comparative purposes:

• RDP, the Ribosomal Database Project;
• SILVA, a ribosomal RNA gene database project.

References

References

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5. (1988-05-01). "Partial phylogeny of the unicellular eukaryotes based on rapid sequencing of a portion of 28S ribosomal RNA". Proceedings of the National Academy of Sciences.
6. (2006). "Reconstructing the early evolution of Fungi using a six-gene phylogeny". Nature.
7. (1997-03-01). "The Strepsiptera Problem: Phylogeny of the Holometabolous Insect Orders Inferred from 18S and 28S Ribosomal DNA Sequences and Morphology". Systematic Biology.
8. (March 2001). "A Combined Molecular Approach to Phylogeny of the Jumping Spider Subfamily Dendryphantinae (Araneae: Salticidae)". Molecular Phylogenetics and Evolution.
9. (2010-03-01). "Molecular phylogeny of Arthrotardigrada (Tardigrada)". Molecular Phylogenetics and Evolution.
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11. Mallatt, Jon. (December 1998). "28S and 18S rDNA sequences support the monophyly of lampreys and hagfishes". Molecular Biology and Evolution.
12. (December 2019). "Computational discovery of hidden breaks in 28S ribosomal RNAs across eukaryotes and consequences for RNA Integrity Numbers". Scientific Reports.
13. (September 30, 2016). "Ribosomal Database Project".
14. (1992-05-11). "The Ribosomal Database Project". Nucleic Acids Research.
15. (2014-01-01). "Ribosomal Database Project: data and tools for high throughput rRNA analysis". Nucleic Acids Research.
16. (2013-01-01). "The SILVA ribosomal RNA gene database project: improved data processing and web-based tools". Nucleic Acids Research.