Sf9 (cells)

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Insect cell line

title: "Sf9 (cells)" type: doc version: 1 created: 2026-02-28 author: "Wikipedia contributors" status: active scope: public tags: ["insect-cell-lines", "spodoptera"] description: "Insect cell line" topic_path: "general/insect-cell-lines" source: "https://en.wikipedia.org/wiki/Sf9_(cells)" license: "CC BY-SA 4.0" wikipedia_page_id: 0 wikipedia_revision_id: 0

::summary Insect cell line ::

::figure[src="https://upload.wikimedia.org/wikipedia/commons/8/84/SF9-AcMNPV.JPG"] ::

::figure[src="https://upload.wikimedia.org/wikipedia/commons/d/dc/SF9_Cells_phase_contrast_Lara_Rudman.jpg" caption="Phase contrast image of SF9 cells"] ::

Sf9 cells, a clonal isolate of Spodoptera frugiperda Sf21 cells (IPLB-Sf21-AE), are commonly used in insect cell culture for recombinant protein production using baculovirus expression system. They were originally established from ovarian tissue. They can be grown in the absence of serum, and can be cultured attached or in suspension.

Sf9 cell lines are widely utilized in the manufacture of recombinant protein antigens, including certain COVID-19 vaccines (like NVX-CoV2373 or ZF2001). Because subunit vaccines contain only specific viral components, they are generally considered safe for use across diverse demographic groups, including individuals with reduced immune function, as well as children and older adults. An additional practical advantage of this vaccine type is its stability, allowing for easier storage and distribution.

Sf9 Rhabdovirus

It has previously been shown that some Sf9 cell lines harbor a negative sense Rhabdovirus called Spodoptera frugiperda rhabdovirus (SfRV). However, not all tested Sf9 cells appear to be infected with this virus. SfRV appears to be insect-specific and does not appear to infect mammalian cell lines.

References

References

  1. (1977). "The establishment of two cell lines from the insect Spodoptera frugiperda (Lepidoptera; Noctuidae)". In Vitro.
  2. Invitrogen. (14 Mar 2017). "Growth and Maintenance of Insect cell lines: User Guide". Invitrogen.
  3. Meng, Fan-Yue. (2021-07-15). "Safety and immunogenicity of a recombinant COVID-19 vaccine (Sf9 cells) in healthy population aged 18 years or older: two single-center, randomised, double-blind, placebo-controlled, phase 1 and phase 2 trials". Signal Transduction and Targeted Therapy.
  4. Ma H, Galvin TA, Glasner DR, Shaheduzzaman S, Khan AS. (2014). "Identification of a novel rhabdovirus in Spodoptera frugiperda cell lines.". J Virol.
  5. Parry R, de Malmanche H, Asgari S. (2021). "Persistent Spodoptera frugiperda rhabdovirus infection in Sf9 cells is not restricted by Wolbachia wMelPop-CLA and wAlbB strains and is targeted by the RNAi machinery.". Virology.
  6. Hashimoto Y, Macri D, Srivastava I, McPherson C, Felberbaum R, Post P. (2017). "Complete study demonstrating the absence of rhabdovirus in a distinct Sf9 cell line.". PLOS ONE.
  7. Maghodia AB, Jarvis DL. (2017). "Infectivity of Sf-rhabdovirus variants in insect and mammalian cell lines.". Virology.

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insect-cell-linesspodoptera