MOPS

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title: "MOPS" type: doc version: 1 created: 2026-02-28 author: "Wikipedia contributors" status: active scope: public tags: ["buffer-solutions", "sulfonic-acids", "4-morpholinyl-compounds"] topic_path: "general/buffer-solutions" source: "https://en.wikipedia.org/wiki/MOPS" license: "CC BY-SA 4.0" wikipedia_page_id: 0 wikipedia_revision_id: 0

| Verifiedfields = changed | Watchedfields = changed | verifiedrevid = 442344970 | ImageFile = Mops is.svg | PIN = 3-(Morpholin-4-yl)propane-1-sulfonic acid | OtherNames = 3-(N-Morpholino)propanesulfonic acid, 3-Morpholinopropanesulfonic acid, 3-N-Morpholino propansulfonic acid, 4-Morpholinepropanesulfonic acid | Section1 = {{Chembox Identifiers | CASNo_Ref = | CASNo = 1132-61-2 | CASNo_Comment = (free acid) | CASNo2_Ref = | CASNo2 = 71119-22-7 | CASNo2_Comment = (sodium salt) | UNII_Ref = | UNII = 273BP63NV3 | PubChem = 70807 | SMILES = C1COCCN1CCCS(=O)(=O)O | PubChem2 = 2723950 | ChemSpiderID_Ref = | ChemSpiderID = 63972 | InChI = 1/C7H15NO4S/c9-13(10,11)7-1-2-8-3-5-12-6-4-8/h1-7H2,(H,9,10,11) | InChIKey = DVLFYONBTKHTER-UHFFFAOYAN | StdInChI_Ref = | StdInChI = 1S/C7H15NO4S/c9-13(10,11)7-1-2-8-3-5-12-6-4-8/h1-7H2,(H,9,10,11) | StdInChIKey_Ref = | StdInChIKey = DVLFYONBTKHTER-UHFFFAOYSA-N | Section2 = {{Chembox Properties | C=7|H=15|N=1|O=4|S=1 | Appearance = | Density = | MeltingPt = | BoilingPt = | Solubility = | Section3 = {{Chembox Hazards | GHSPictograms = | GHSSignalWord = Warning | HPhrases = | PPhrases = | MainHazards = | FlashPt = | AutoignitionPt = | ExternalSDS =

MOPS (3-(N-morpholino)propanesulfonic acid) is a buffer introduced in the 1960s, one of the twenty Good's buffers. It is a structural analog to MES, and like MES, its structure contains a morpholine ring. HEPES is a similar pH buffering compound that contains a piperazine ring. With a pKa of 7.20, MOPS is an excellent buffer for many biological systems at near-neutral pH.

Applications

MOPS is frequently used as a buffering agent in biology and biochemistry. It has been tested and recommended for polyacrylamide gel electrophoresis. Usage above 20 mM in mammalian cell culture work is not recommended. MOPS buffer solutions become discolored (yellow) over time, but reportedly slight discoloration does not significantly affect the buffering characteristics.

References

References

  1. (1966). "Hydrogen Ion Buffers for Biological Research". Biochemistry.
  2. (1981). "A new discontinuous buffer system for the electrophoresis of cationic proteins at near-neutral pH". Analytical Biochemistry.
  3. (1971). "Buffer Combinations for Mammalian Cell Culture". Science.
  4. "Boston BioProducts - MOPS Buffer (1 M, pH 7.4)".

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buffer-solutionssulfonic-acids4-morpholinyl-compounds