Surf Wiki
Save to docs
general/nucleosides

From Surf Wiki (app.surf) — the open knowledge base

5-Ethynyl-2'-deoxyuridine

5-Ethynyl-2′-deoxyuridine (EdU) is a thymidine analogue which is incorporated into the DNA of dividing cells. EdU is used to assay DNA synthesis in cell culture and detect cells in embryonic, neonatal and adult animals which have undergone DNA synthesis. Whilst at high doses it can be cytotoxic, this molecule is now widely used to track proliferating cells in multiple biological systems.

EdU-labelling allows cells to be isolated without denaturing DNA, allowing researchers to determine the transcriptional profile of cells. This approach has been used to assess transcription in neuronal cells and tissues that have recently divided either in vitro or in vivo.

Detection

EdU is labeled and detected with an azide molecule (most commonly fluorescent azides) through Cu(I)-catalyzed azide–alkyne cycloaddition (CuAAC) click chemistry. Unlike the commonly used bromodeoxyuridine (BrdU), EdU detection requires no heat or acid treatment.

Toxicity

EdU incorporated into DNA induces DNA damage through the formation of interstrand crosslinks. These are detected by the cell during DNA replication, which is reflected by phosphorylation of histone H2AX, arrest in the cell cycle progression, and apoptosis.

References

References

  1. (January 2016). "Isolating dividing neural and brain tumour cells for gene expression profiling". Journal of Neuroscience Methods.
  2. (January 2016). "Transcriptional profiling of dividing tumor cells detects intratumor heterogeneity linked to cell proliferation in a brain tumor model". Molecular Oncology.
  3. (September 2017). "A method for isolating cortical interneurons sharing the same birthdays for gene expression studies". Experimental Neurology.
  4. (February 2009). "EdU, a new thymidine analogue for labelling proliferating cells in the nervous system". Journal of Neuroscience Methods.
  5. (September 2011). "Thymidine analogues for tracking DNA synthesis". Molecules.
  6. (2015-02-11). "A fatal combination: a thymidylate synthase inhibitor with DNA damaging activity". PLOS ONE.
  7. (November 2013). "DNA damage signaling, impairment of cell cycle progression, and apoptosis triggered by 5-ethynyl-2'-deoxyuridine incorporated into DNA". Cytometry. Part A.
Info: Wikipedia Source

This article was imported from Wikipedia and is available under the Creative Commons Attribution-ShareAlike 4.0 License. Content has been adapted to SurfDoc format. Original contributors can be found on the article history page.

nucleosides staining-dyes pyrimidinediones ethynyl-compounds
Want to explore this topic further?

Ask Mako anything about 5-Ethynyl-2'-deoxyuridine — get instant answers, deeper analysis, and related topics.

Research with Mako

Free with your Surf account

Content sourced from Wikipedia, available under CC BY-SA 4.0.

This content may have been generated or modified by AI. CloudSurf Software LLC is not responsible for the accuracy, completeness, or reliability of AI-generated content. Always verify important information from primary sources.

Report