Silverquant

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title: "Silverquant" type: doc version: 1 created: 2026-02-28 author: "Wikipedia contributors" status: active scope: public tags: ["gene-expression", "bioinformatics", "dna", "microarrays"] description: "Labeling and detection method for DNA microarrays or protein microarrays" topic_path: "science/biology" source: "https://en.wikipedia.org/wiki/Silverquant" license: "CC BY-SA 4.0" wikipedia_page_id: 0 wikipedia_revision_id: 0

::summary Labeling and detection method for DNA microarrays or protein microarrays ::

Silverquant is a labeling and detection method for DNA microarrays or protein microarrays. A synonym is colorimetric detection. In contrast to the classical signal detection on microarrays by using fluorescence, the colorimetric detection is more sensitive and ozone-stable.

Chemical reaction

The probe to be detected is labeled with some biotin-molecules. After incubation with a gold-coupled anti-biotin conjugate, silver nitrate and a reducing agent are added. The reaction starts whereas the gold particle serves as a starting point for the silver precipitation. The reaction needs to be stopped after a specific time. The constant reaction time is essential to obtain comparable results.

Detection

The silver-stained spots on the microarray are clearly visible. By using a transmission microarray scanner, the signals are transformed into digital values which are finally available as an image file.

References

Alexandre I et al. Anal Biochem. 2001 Aug 1;295(1):1-8.

::callout[type=info title="Wikipedia Source"] This article was imported from Wikipedia and is available under the Creative Commons Attribution-ShareAlike 4.0 License. Content has been adapted to SurfDoc format. Original contributors can be found on the article history page. ::