Nucleic acid methods
Techniques used to study nucleic acids
title: "Nucleic acid methods" type: doc version: 1 created: 2026-02-28 author: "Wikipedia contributors" status: active scope: public tags: ["genetics-techniques", "molecular-biology", "nucleic-acids"] description: "Techniques used to study nucleic acids" topic_path: "science/biology" source: "https://en.wikipedia.org/wiki/Nucleic_acid_methods" license: "CC BY-SA 4.0" wikipedia_page_id: 0 wikipedia_revision_id: 0
::summary Techniques used to study nucleic acids ::
Nucleic acid methods are the techniques used to study nucleic acids: DNA and RNA.
Purification
- DNA extraction
- Phenol–chloroform extraction
- Minicolumn purification
- RNA extraction
- Boom method
- Synchronous coefficient of drag alteration (SCODA) DNA purification
Quantification
- Abundance in weight: spectroscopic nucleic acid quantitation
- Absolute abundance in number: real-time polymerase chain reaction (quantitative PCR)
- High-throughput relative abundance: DNA microarray
- High-throughput absolute abundance: serial analysis of gene expression (SAGE)
- Size: gel electrophoresis
Synthesis
- De novo: oligonucleotide synthesis
- Amplification: polymerase chain reaction (PCR), loop-mediated isothermal amplification (LAMP), transcription-mediated amplification (TMA)
Kinetics
- Multi-parametric surface plasmon resonance
- Dual-polarization interferometry
- Quartz crystal microbalance with dissipation monitoring (QCM-D)
Gene function
Other
- Bisulfite sequencing
- DNA sequencing
- Expression cloning
- Fluorescence in situ hybridization
- Lab-on-a-chip
- Comparison of nucleic acid simulation software
- Northern blot
- Nuclear run-on assay
- Radioactivity in the life sciences
- Southern blot
- Differential centrifugation (sucrose gradient)
- Toeprinting assay
- Several bioinformatics methods, as seen in list of RNA structure prediction software
References
References
- (2025). "Challenging biological samples and strategies for DNA extraction". Journal of Investigative Medicine.
- (23 September 2014). "A universal molecular translator for non-nucleic acid targets that enables dynamic DNA assemblies and logic operations". Chem. Commun..
- (7 March 2014). "An impedimetric study of DNA hybridization on paper-supported inkjet-printed gold electrodes". Nanotechnology.
- (2005). "Dual polarization interferometry size and density characterisation of DNA immobilisation and hybridisation". Biosensors and Bioelectronics.
- Dixon, Matthew C.. (July 2008). "Quartz Crystal Microbalance with Dissipation Monitoring: Enabling Real-Time Characterization of Biological Materials and Their Interactions". Journal of Biomolecular Techniques.
- Hannon, Gregory J.. (July 2002). "RNA interference". Nature.
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